bacteria strain

Bacillus subtilis

Bacillus subtilis

Biostimulant & Soil Inoculant for Plant Health, Resilience & Nutrient Efficiency

Bacillus subtilis is a spore-forming, plant growth-promoting rhizobacterium (PGPR) widely applied in agriculture to enhance soil function, plant growth, and biological resilience. Through natural metabolite production, nutrient mobilization, and immune priming, this microbial inoculant contributes to healthier plants and more productive soils. Our Bacillus subtilis soil probiotic is ideal for use across crop types and climates, particularly in stress-prone environments or regenerative systems.

  • Supports natural plant defense responses (SAR/ISR)
  • Promotes root growth and early crop establishment
  • Enhances phosphorus and micronutrient availability
  • Contributes to soil microbial balance and biodiversity
  • Improves plant tolerance to drought, salinity, and heat stress
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Activation of Plant Defense Pathways (SAR & ISR)

Bacillus subtilis helps plants activate their own internal defense systems against biotic and abiotic stress.

  • Induces Systemic Acquired Resistance (SAR) via salicylic acid signaling pathways
  • Triggers Induced Systemic Resistance (ISR) via jasmonic acid and ethylene pathways
  • Stimulates production of natural defense enzymes (e.g., peroxidase, chitinase, β-glucanase)

Root Health & Plant Growth Promotion

B. subtilis is a versatile soil inoculant that actively supports strong root systems, helping crops establish faster and grow more vigorously.

  • Produces indole-3-acetic acid (IAA) and other phytohormones that promote root elongation and branching
  • Improves root hair development for nutrient and water access
  • Enhances seed germination speed and early seedling vigor
  • Increases chlorophyll synthesis, boosting photosynthetic efficiency and biomass accumulation

Enhanced Nutrient Mobilization & Uptake

This strain plays an essential role as one of the leading soil fertility microbes, especially in low-input or nutrient-deficient soils. It unlock nutrients from the soil, improving plant availability of both macronutrients and micronutrients.

  • Solubilizes phosphate from insoluble forms like tricalcium phosphate, iron phosphate, and aluminum phosphate
  • Produces siderophores that chelate and mobilize iron (Fe³⁺) in calcareous and alkaline soils
  • Improves uptake of key micronutrients:
    • Zinc (Zn) – critical for enzyme activation and hormone balance
    • Manganese (Mn) – supports photosynthesis and disease resistance
    • Copper (Cu) – involved in lignin synthesis and stress signaling
    • Magnesium (Mg) – essential for chlorophyll and energy metabolism
    • Sulfur (S) – vital for amino acid formation and protein synthesis

Abiotic Stress Mitigation

By improving root function and physiological balance, this microbial inoculant enhances plant resilience to a wide range of environmental challenges.

  • Drought stress – Promotes deeper rooting and increases water-use efficiency
    Salinity stress – Maintains ion balance (Na⁺/K⁺ ratio) and osmotic adjustment
  • Heat stress – Reduces oxidative damage by boosting antioxidant enzyme systems

Soil Microbiome Enhancement & Biological Resilience

Recognized as one of the best microbes for soil health, B. subtilis contributes to a healthier soil microbiome by establishing early, occupying space, and producing beneficial metabolites.

  • Produces lipopeptides (surfactin, fengycin, iturin) that influence soil microbial dynamics
  • Competes for space and resources in the root zone (competitive exclusion)
  • Supports soil aggregation, moisture retention, and decomposition of crop residues

Application Flexibility & Cropping Compatibility

Widely used as a microbial biofertilizer in organic farming systems, Bacillus subtilis is easy to integrate into existing fertility and protection protocols.

  • Can be applied via seed treatment, in-furrow application, drip irrigation, fertigation, or foliar spray
  • Compatible with most liquid fertilizers, humic acids, and other microbial inoculants
  • Safe for use in conventional, no-till, and hydroponic systems
  • Effective across crops: row crops, vegetables, orchards, turf, and greenhouse production

Why Choose Our Bacillus subtilis?

High CFU Potency – 200 billion CFU/g for robust root colonization and reliable field activity
Defense-Priming PGPR – Supports SAR/ISR pathways without chemical inputs
Multinutrient Mobilizer – Unlocks phosphorus, iron, zinc, manganese, and more
Stress Resilience Booster – Enhances crop tolerance to drought, salt, heat, and toxic elements
Soil-Friendly and Sustainable – Improves soil health while supporting long-term productivity

Proudly Made in the USA – Manufactured by Applied Biotech Industries under strict quality control

At Applied Biotech Industries, we are a U.S.-based manufacturer and fermentation producer of high-performance microbial fertilizers for agriculture. Our Bacillus subtilis strain is available in bulk or custom packaging formats and is ideal for distributors, input suppliers, and large-scale growers looking to buy biofertilizers online, order agricultural microbes, or source premium microbial inoculants for soil and crop health. 

Whether you're looking for beneficial microbes for plants, wholesale microbial fertilizers, or a reliable supplier of agricultural microbial strains, we provide consistent, high-CFU formulations backed by strict quality control. Contact us today to buy agricultural microbes that deliver proven performance in the field.

Concentration (CFU/g):

  • 200 billion (2.0 × 10¹¹ CFU/g) dry powder
  • Custom concentrations available upon request

Particle Size (Mesh):

  • Passes through 100 mesh sieve

Packaging Options:

  • 22 lb (10 kg) pails
  • 44 lb (20 kg) pails
  • 340 lb (155 kg) drums
  • Smaller custom packaging available on request

Shelf-life:

  • 1.5 years at room temperature
  • 2+ years if refrigerated

Storage Recommendations:

  • Store in a cool, dry location away from direct sunlight.
  • Keep container tightly sealed after each use.

Soil Drench / In-Furrow Application

Dosage:

  • 100–200 grams per acre
  • 250–500 grams per hectare

Frequency:

  • At planting or during early root development
  • Reapply every 4–8 weeks as needed

Application Method:

  1. Dissolve thoroughly in water (use enough water to reach the root zone)
  2. Use an agitation tank or manual stirring to maintain microbial suspension
  3. Apply through drip irrigation lines, micro-sprayers, or fertigation systems, positioning flow as close to the root zone as possible
  4. Flush the system after application to prevent clogging or residue buildup

When tank-mixing with fertilizers:

  • Dilute fertilizer fully in water first before adding Bacillus subtilis
  • Do not mix with herbicides, fungicides, bactericides, or pesticides


Fertigation / Drip Irrigation

Dosage:

  • 50–150 grams per acre
  • 125–375 grams per hectare

Frequency:

  • Apply during early root establishment or transplanting
  • Reapply every 2–4 weeks during active growth stages as needed

Application Method:

  1. Dissolve thoroughly in water (use enough water to reach the root zone)
  2. Use an agitation tank or manual stirring to maintain microbial suspension
  3. Apply through drip irrigation lines, micro-sprayers, or fertigation systems, positioning flow as close to the root zone as possible
  4. Flush the system after application to prevent clogging or residue buildup


Foliar Spray

Dosage:

  • 50–100 grams per acre
  • 125–250 grams per hectare
  • Dilution: 2.5–5 g per 10 liters of water (0.25–0.5%)

Frequency:

  • Begin at vegetative or pre-flowering stages
  • Reapply every 7–14 days under high stress or disease pressure

Application Method:

  1. Apply in the early morning or evening to reduce UV exposure
  2. Use a non-ionic surfactant or wetting agent for improved adhesion
  3. Ensure thorough coverage of foliage, including undersides of leaves
  4. Agitate spray solution continuously during application


Seed Treatment

Dosage:

  • 2–5 grams per 100 lbs (45 kg) of seed

Frequency:

  • Single application before planting

Application Method:

  1. Mix thoroughly with a sticking agent (e.g., sugar solution, gum arabic)
  2. Coat seeds evenly, ensuring full surface coverage
  3. Allow seeds to dry gently in the shade before sowing

Q: What is Bacillus subtilis and how does it support crop growth?

A: Bacillus subtilis is a naturally occurring, spore-forming plant growth-promoting rhizobacterium (PGPR). It functions as a biostimulant, biofertilizer, and soil inoculant, enhancing nutrient use efficiency, stimulating root development, and priming natural plant defenses (SAR and ISR). As a trusted agricultural microbial strain, it improves soil health and crop resilience in a wide range of farming systems.

Q: Where can I buy Bacillus subtilis in bulk?

A: Applied Biotech Industries is a direct manufacturer and fermentation producer of Bacillus subtilis. We supply bulk quantities from our Wisconsin-based production facility. Our wholesale packaging options include:

  • 22 lb (10 kg) pails
  • 44 lb (20 kg) pails
  • 340 lb (155 kg) drums

We serve large-scale farms, input manufacturers, and distributors looking to buy agricultural microbes, wholesale microbial fertilizers, or beneficial soil bacteria.

Q: Does Applied Biotech Industries manufacture Bacillus subtilis directly?

A: Yes. We produce Bacillus subtilis in-house using controlled fermentation for high CFU counts, product stability, and consistent field performance. We’re a trusted microbial fertilizers manufacturer supplying customers across organic, regenerative, and conventional agriculture.

Q: How is Bacillus subtilis applied in agriculture?

A: Bacillus subtilis can be applied using several common methods:

  • Seed treatment for early root colonization
    In-furrow application at planting
  • Soil drench or drip irrigation for root zone delivery
  • Foliar spray to activate natural defense responses
    It is compatible with many liquid fertilizers, organic inputs, and microbial blends.

Q: What nutrients does Bacillus subtilis help plants access?

A: Bacillus subtilis improves plant access to:

  • Phosphorus (P) – by solubilizing mineral-bound phosphates
  • Iron (Fe) – through siderophore chelation
  • Micronutrients such as zinc (Zn), manganese (Mn), copper (Cu), magnesium (Mg), sulfur (S), and calcium (Ca)

This makes it an effective component in microbial biofertilizer programs focused on nutrient efficiency.

Q: Can Bacillus subtilis be used in certified organic farming?

A: Yes. Bacillus subtilis is a non-GMO, naturally occurring strain used widely in organic agriculture. It improves soil biodiversity, supports nutrient cycling, and enhances plant health without synthetic inputs. However, always confirm compliance with your organic certification body.

Q: Which crops benefit from Bacillus subtilis applications?

A: B. subtilis supports a wide range of crops, including:

  • Row crops – corn, wheat, soybeans, sorghum
  • Vegetables & fruits – tomatoes, lettuce, strawberries, citrus
  • Perennials – grapes, apples, berries
  • Specialty & high-value crops – herbs, ornamentals, greenhouse vegetables.

Q: Is Bacillus subtilis compatible with fertilizers and agricultural inputs?

A: Yes. Bacillus subtilis works well with most liquid fertilizers, humic substances, and organic inputs. When tank-mixing, dilute fertilizers in water before adding microbes. Avoid direct mixing with chemical pesticides unless compatibility has been confirmed.

Q: Is Bacillus subtilis safe for the environment?

A: Absolutely. Bacillus subtilis is a beneficial soil microbe that promotes sustainable agriculture. It improves soil fertility, reduces chemical input needs, and supports long-term productivity without environmental harm.

Q: Does Applied Biotech offer wholesale, private-label, or custom supply options?

A: Yes. We provide wholesale agricultural microbes, private-label biofertilizers, and custom formulations for agribusiness clients. Whether you need microbial inoculants for large-scale production, bulk soil probiotics, or fermentation-based formulations, we offer full technical support and flexible logistics.

1. Harnessing Plant Growth-Promoting Rhizobacteria, Bacillus subtilis, to Mitigate Salt-Induced Damage in Rice (Oryza sativa L.)
🔗
Read the study

Key Findings:

  • Application of B. subtilis mitigated salt-induced damage in rice.
  • Enhanced growth parameters and stress tolerance observed in treated plants.
  • Improved antioxidant activity and ionic balance under saline conditions.

2. Evaluation of a Formulation of Bacillus subtilis for Control of Phytophthora Blight of Bell Pepper
🔗 Read the study

Key Findings:

  • B. subtilis formulation effectively controlled Phytophthora blight in bell pepper.
  • Demonstrated potential as a biocontrol agent in greenhouse and field conditions.
  • Increased plant survival and reduced disease severity across trials.

3. Bacillus subtilis-Mediated Abiotic Stress Tolerance in Plants
🔗 Read the study

Key Findings:

  • B. subtilis enhances plant tolerance to various abiotic stresses, including drought and salinity.
  • Mechanisms include modulation of stress-responsive genes and antioxidant enzyme activities.
  • Helps maintain physiological homeostasis under environmental pressure.

4. Biocontrol of Bacterial Wilt Disease in Tomato Using Bacillus subtilis R31
🔗 Read the study

Key Findings:

  • B. subtilis R31 significantly reduced the incidence of bacterial wilt in tomato.
  • Highlighted as an effective biological control agent against Ralstonia solanacearum.
  • Stimulated systemic resistance and supported rhizosphere microbial balance.

5. Biocontrol Mechanisms of Bacillus: Improving the Efficiency of Green Agriculture
🔗 Read the study

Key Findings:

  • Detailed mechanisms by which Bacillus species, including B. subtilis, control plant diseases.
  • Emphasized the role of antimicrobial compound production and induced systemic resistance.
  • Supports integration into sustainable and low-input farming systems.

6. Bacillus subtilis: A Plant-Growth Promoting Rhizobacterium That Also Impacts Biotic Stress
🔗 Read the study

Key Findings:

  • B. subtilis promotes plant growth and enhances resistance to biotic stresses.
  • Functions through phytohormone production, nutrient solubilization, and pathogen suppression.
    Produces VOCs and bioactive peptides that influence the root microbiome.

7. Bacillus subtilis Impact on Plant Growth, Soil Health, and Environment
🔗 Read the study

Key Findings:

  • B. subtilis contributes to improved plant growth and soil health.
  • Plays a role in conferring biotic and abiotic stress tolerance to plants.
  • Enhances rhizosphere microbial diversity and organic matter turnover.

8. Bacillus subtilis ER-08: A Multifunctional Plant Growth-Promoting Rhizobacterium
🔗 Read the study

Key Findings:

  • B. subtilis ER-08 enhances plant growth and stress tolerance.
  • Demonstrated potential as a bio-inoculant for sustainable agriculture.
  • Solubilizes phosphate and produces siderophores for micronutrient mobilization.

9. Bacillus subtilis CH13: A Highly Effective Biocontrol Agent for the Integrated Management of Plant Diseases
🔗 Read the study

Key Findings:

  • B. subtilis CH13 effectively controls various plant diseases.
  • Supports integrated disease management strategies in agriculture.
  • Consistent performance in both greenhouse and open-field trials.

10. Bacillus subtilis Ameliorates Water Stress Tolerance in Maize and Common Bean
🔗 Read the study

Key Findings:

  • B. subtilis application improved drought tolerance in maize and common bean.
  • Enhanced physiological and biochemical responses under water-deficit conditions.
  • Increased water use efficiency and maintained plant biomass under stress.

Inquire about this strain

Address

Applied Biotech Industries 520 Progress Drive Belgium, WI 53004

Contact Info

info@abimicrobes.com
(262) 285-4390

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