Bacillus methylotrophicus

Root-Zone Probiotic for Nutrient Mobilization, Rhizosphere Balance & Plant Resilience

Bacillus methylotrophicus is a spore-forming, fast-colonizing plant growth-promoting rhizobacterium (PGPR) known for its ability to colonize roots, solubilize nutrients, and support beneficial microbial communities in the rhizosphere. Known for its robust production of organic acids, lipopeptides, and phytohormones, this strain contributes to early plant establishment, improved nutrient efficiency, and enhanced resilience under abiotic stress. Its durable spore-based formulation ensures long shelf life, storage stability, and compatibility with a wide range of biological inputs.

Produces phytohormones and organic acids that promote root elongation, seedling vigor, and nutrient uptake efficiency
Solubilizes phosphorus, potassium, and micronutrients such as iron and zinc for improved plant access
Rapidly colonizes root surfaces and forms biofilms that support beneficial microbial communities in the soil
Enhances plant tolerance to salinity, drought, and low-fertility soils through metabolite-driven stress modulation
Compatible with compost teas, biological blends, and microbial formulations targeting soil health and input efficiency

Buy Now / Request Sample

Microbial Balance and Natural Antimicrobial Metabolites

As a beneficial soil probiotic and microbial inoculant, Bacillus methylotrophicus enhances root zone health through natural metabolite production and competitive colonization.

Produces lipopeptides, enzymes, and volatile compounds that help shape a balanced microbial community in the soil
Rapidly colonizes root surfaces and surrounding soil, supporting beneficial organisms through competitive microbial activity
Stimulates the plant’s natural defense signaling pathways, contributing to resilience under environmental and biological stress — without functioning as a synthetic pesticide

Enhanced Plant Growth and Establishment

Functions as a soil fertility microbe that promotes vigorous early development and consistent crop performance through microbial hormone activity.

Produces auxins, cytokinins, and gibberellins to stimulate root elongation, shoot growth, and plant vigor
Enhances seed germination, seedling establishment, and uniform emergence across a variety of crops
Supports biomass accumulation and overall productivity throughout the growing season

Nutrient Solubilization and Uptake

Acts as a beneficial soil microbe to improve the bioavailability of essential nutrients through microbial acidification and enzymatic activity.

Solubilizes phosphorus and potassium, increasing nutrient accessibility in the root zone
Secretes organic acids and enzymes that mobilize micronutrients such as iron, zinc, and manganese
Enhances root nutrient uptake efficiency under diverse soil conditions

Soil Health, Structure, and Organic Matter Recycling

By contributing to organic matter breakdown and soil aggregation, Bacillus methylotrophicus is an organic farming microbe that improves overall soil function and sustainability.

Decomposes crop residues and organic material, supporting nutrient cycling and microbial activity
Promotes soil aggregation, porosity, and water retention, enhancing physical soil quality
Supports long-term soil fertility and performance in conventional, regenerative, and no-till systems

Abiotic Stress Resilience

This strain helps plants withstand environmental challenges by stabilizing metabolic responses and producing protective compounds.

Improves plant tolerance to salinity, drought, and low-fertility soils through microbial signaling
Produces volatile organic compounds and hormone-like metabolites that buffer plant stress responses
Contributes to crop stability and performance under changing climatic conditions

Root Colonization and Biofilm Formation

Bacillus methylotrophicus forms structured microbial communities around roots to support nutrient access and physical protection.

Establishes dense populations at the rhizoplane through efficient colonization mechanisms
Forms biofilms that enhance nutrient and water uptake while shielding roots from environmental extremes
Supports microbial persistence and consistency in field applications

Why Choose Our Bacillus methylotrophicus

High CFU Count for Reliable Field Performance – Manufactured at 300 billion CFU/g, our Bacillus methylotrophicus formulation delivers high spore viability and rapid root colonization for consistent results across crops, soils, and climates.
Phytohormones and VOCs for Early Plant Vigor – Produces auxin-like compounds and beneficial volatile organic compounds (VOCs) that enhance seed germination, root elongation, and early nutrient uptake.
Organic Acid Production for Nutrient Solubilization – Secretes gluconic acid and other organic acids that mobilize phosphorus, potassium, and micronutrients, improving nutrient bioavailability in diverse soil types.
Biofilm Formation and Rhizosphere Competitiveness – Establishes robust microbial populations on root surfaces, forming biofilms that support microbial community balance and protect root zones under environmental stress.
Supports Soil Health and Organic Matter Turnover – Contributes to the decomposition of plant residues and organic material, improving soil fertility, structure, and long-term nutrient cycling.
Broad Compatibility with Biological Inputs – Integrates seamlessly with compost teas, Trichoderma, mycorrhizal fungi, and biofertilizer blends used in organic, regenerative, and low-input farming systems.
Trusted U.S. Manufacturer of Agricultural Microbial Inputs – We supply high-quality, spore-forming Bacillus methylotrophicus strains in bulk, wholesale, and private-label formats for agricultural and soil applications.

Looking to buy beneficial agricultural microbes like Bacillus methylotrophicus? This soil probiotic strain is an excellent choice among microbial inoculants and biofertilizers, enhancing crop health through improved nutrient solubilization, increased nitrogen availability, and balanced microbial diversity. As a potent plant growth-promoting rhizobacteria (PGPR), Bacillus methylotrophicus rapidly colonizes roots, forming biofilms that support beneficial soil microbes including beneficial soil bacteria and beneficial soil fungi.

Trusted in sustainable, regenerative, and organic agriculture, our microbial strains improve soil fertility, resilience, and overall crop performance. Whether you're sourcing bulk microbial fertilizers, private-label microbial inputs for plants, or specialized soil inoculants, Applied Biotech Industries is your U.S.-based partner. Discover our portfolio of beneficial microbes, including powerful phosphate-solubilizing bacteria, potassium-solubilizing bacteria, and soil health microbes tailored specifically for your farming needs.

Concentration (CFU/g):

300 billion (3.0 × 10¹¹) CFU/g dry powder
Custom concentrations available upon request

Particle Size (Mesh):

Passes through 100 mesh sieve

Packaging Options:

22 lb (10 kg) pails
44 lb (20 kg) pails
340 lb (155 kg) drums
Smaller custom packaging available on request

Shelf-life:

1.5 years at room temperature
2+ years refrigerated

Storage Recommendations:

Store in a cool, dry location away from direct sunlight.
Reseal container tightly after each use.

Soil Application (Drip, Drench, or Fertigation)

Dosage:

25–50 grams per acre (65–130 grams per hectare)

Frequency:

Apply during early root establishment or transplanting
Reapply every 2–4 weeks during active growth stages as needed

Application Method:

Dissolve thoroughly in water (use enough water to reach the root zone)
Use an agitation tank or manual stirring to maintain microbial suspension
Apply through drip irrigation lines, micro-sprayers, or fertigation systems, positioning flow as close to the root zone as possible
Flush the system after application to prevent clogging or residue buildup

Do not tank mix with herbicides, fungicides, bactericides, or chemical pesticides.

In-Furrow

Dosage:

5–10 grams per acre (12.5–25 grams per hectare)

Frequency:

Apply once at planting to target root initiation and early growth stages.

Application Method:

Dissolve microbial powder thoroughly in water.
Apply directly into the seed furrow or planting trench at seeding time.
If using irrigation systems for delivery, ensure solution contacts the root zone.
Maintain agitation to prevent settling.

When tank-mixing with fertilizers:

Dilute fertilizer fully in water first before adding microbes
Do not mix with herbicides, fungicides, bactericides, or pesticides

Foliar Spray

Dosage:

12.5–25 grams per acre (30–60 grams per hectare)

Frequency:

Begin at vegetative or pre-flowering stages
Reapply every 7–14 days, especially during periods of high stress or disease pressure

Application Method:

Apply in the early morning or evening to reduce UV exposure
Use a non-ionic surfactant or wetting agent for improved adhesion
Ensure thorough coverage of foliage, including undersides of leaves
Agitate spray solution continuously during application

Seed Treatment

Dosage:

2–4 grams per kg of seed

Frequency:

Single application before planting

Application Method:

Mix thoroughly with a sticking agent (e.g., sugar solution, gum arabic)
Coat seeds evenly, ensuring full surface coverage
Allow seeds to dry gently in the shade before sowing

Composting or Organic Matter Amendment

Dosage:

2.5–5 grams per cubic meter of compost, organic substrate, or potting mix

Frequency:

Single application before use or at the beginning of composting/soil blending

Application Method:

For composting, dissolve powder in water. Then spray piles and turn to evenly distribute product through the piles/windrows.
For organic matter amendment, evenly mix the dry microbial powder growing media during turning or mixing

‍

Q: What is Bacillus methylotrophicus and how is it used in agriculture?

A: Bacillus methylotrophicus is a spore-forming, plant growth-promoting rhizobacterium (PGPR) widely used as a microbial inoculant in regenerative, organic, and conventional farming systems. It colonizes the rhizosphere, enhances nutrient solubilization, and supports microbial balance in the soil. It’s a key component in many microbial fertilizers, compost amendments, and soil health products designed to boost crop resilience and productivity.

Q: How does Bacillus methylotrophicus improve crop performance without acting as a pesticide?

A: B. methylotrophicus enhances plant growth and soil function by producing organic acids, enzymes, and phytohormones that stimulate root development, nutrient uptake, and abiotic stress tolerance. It strengthens microbial diversity and biofilm formation in the root zone—without acting as a pesticide or requiring EPA registration, making it fully compatible with organic farming microbes programs.

Q: What sets Bacillus methylotrophicus apart from other soil microbes?

A: Unlike vegetative bacteria that degrade quickly in storage or high-heat environments, B. methylotrophicus forms stable spores, allowing for long shelf life, easy handling, and integration into dry or liquid biofertilizer formulations. Its combination of spore durability, root colonization efficiency, and metabolite production makes it one of the best microbes for soil health.

Q: Where can I buy Bacillus methylotrophicus or other agricultural microbial strains?

A: Applied Biotech Industries offers agricultural bacteria for sale, including high-CFU Bacillus methylotrophicus available in bulk, wholesale, and private-label formats. Whether you're looking to buy microbial inoculants online or need a dependable microbial fertilizers manufacturer, we provide fermentation-controlled, spore-forming strains ready for integration into professional agricultural inputs.

Q: Is Bacillus methylotrophicus suitable for organic and sustainable agriculture?

A: Yes. B. methylotrophicus is frequently used in organic farming programs, microbial consortia, and compost-based inputs. It aligns with soil-building strategies focused on nutrient cycling, microbial diversity, and reduced reliance on synthetic fertilizers.

Q: What crops benefit most from Bacillus methylotrophicus inoculation?

A: B. methylotrophicus supports a wide variety of crops, including vegetables, grains, legumes, fruit trees, and greenhouse systems. It is especially valuable during early plant development stages for enhancing germination, seedling vigor, and root system establishment—making it a go-to microbial fertilizer for plants across soil types.

Q: Can Bacillus methylotrophicus be blended with other beneficial microbes?

A: Absolutely. B. methylotrophicus is highly compatible with Trichoderma, Pseudomonas, mycorrhizal fungi, and other beneficial microbes for plants. It integrates well into multi-strain products targeting nutrient cycling, soil resilience, and root zone stability.

Q: How is Bacillus methylotrophicus applied on farms?

A: This strain can be applied through in-furrow treatment, drip irrigation, soil drench, or blended into compost teas and foliar sprays. It dissolves well in water and remains stable under most field mixing conditions. As a versatile microbial inoculant, it fits seamlessly into organic and conventional application programs.

Q: Is Bacillus methylotrophicus shelf-stable and viable over time?

A: Yes. Thanks to its spore-forming nature, B. methylotrophicus maintains high viability during storage. When kept in a cool, dry environment, it remains viable for at least 1.5 years. This makes it ideal for long-term supply chains and wholesale microbial fertilizers distribution.

Q: What packaging formats are available for Bacillus methylotrophicus?

A: We offer Bacillus methylotrophicus in 10 kg (22 lb) and 20 kg (44 lb) pails, as well as 155 kg (340 lb) drums. Custom CFU concentrations and packaging options are available for distributors, input producers, and ag retailers looking to develop their own private-label microbial products.

Q: Why choose Applied Biotech Industries for Bacillus methylotrophicus?

A: We are a U.S.-based producer of microbial fertilizers, specializing in high-CFU, spore-forming strains like Bacillus methylotrophicus. Our products are trusted by input manufacturers, retailers, and regenerative growers alike. We offer full-service support, reliable fermentation-based production, and custom packaging options for companies seeking top-quality beneficial microbes for plants in bulk or wholesale formats.

Safety Data Sheet (SDS)

1. Bacillus methylotrophicus VL‑10 Enhances Tomato Cold Stress Tolerance
🔗 Read the study

Key Findings:

Inoculation of tomato seedlings with B. methylotrophicus VL‑10 significantly improved plant recovery from low-temperature shock (~15 °C/8 °C cycles).
Treated plants showed improved leaf chlorophyll (SPAD) and photosynthetic efficiency (Fv/Fm), quicker flowering, and restoration of growth post-stress

2. Bacillus methylotrophicus M4‑1 Alleviates Salt Stress in Winter Wheat
🔗 Read the study

Key Findings:

Strain M4‑1 reduced rhizosphere pH and electrical conductivity (EC), and decreased Na⁺ accumulation in roots and leaves.
It enhanced uptake of K⁺ (up to ~13–14%) and Mg²⁺ (~20%), improving wheat biomass and salt tolerance

3. Bacillus methylotrophicus M4‑96 Promotes Strawberry Growth and Disease Defense
🔗 Read the study

Key Findings:

In vitro and volatile exposure experiments showed M4‑96 stimulates strawberry seedling growth and induces callose deposition, reducing Botrytis cinerea infection severity

4. Bacillus methylotrophicus KE2 Produces Gibberellins in Lettuce
🔗 Read the study

Key Findings:

KE2 strain synthesized gibberellins, fostering lettuce growth and enhancing key nutritional metabolites and food quality

5. Bacillus methylotrophicus DD‑1 Boosts Rice Growth via Multitrait PGPR Activity
🔗 Read the study

Key Findings:

DD‑1 exhibited strong potassium solubilization (1.18 mg/L), produced IAA (87 mg/L), gibberellin (26 mg/L), and siderophores (52%).
Rice seedlings showed increases in root/shoot length, biomass, tiller count, and enhanced root colonization

6. Bacillus methylotrophicus as a Biofertilizer in Tomato Plug Seedlings
🔗 Read the study

Key Findings:

Applied at various dosages, B. methylotrophicus improved tomato seedling height, stem thickness, leaf area, and dry matter accumulation in transplants

7. Mechanisms of Nutrient Solubilization and Phytohormone Production by Bacillus spp.
🔗 Read the study

Key Findings:

Highlights how Bacillus species (including methylotrophicus) solubilize phosphorus and micronutrients via organic acids, produce auxins, cytokinins, gibberellins, ACC deaminase, and siderophores, and form biofilms to support plant nutrient access

Bacillus methylotrophicus