Bacillus subtilis

Advanced Biofertilizer for Plant Health & Nutrient Uptake

Our Bacillus subtilis powder biofertilizer is a high-CFU, spore-forming plant growth-promoting rhizobacterium (PGPR) used in agriculture to enhance soil health, crop performance, and abiotic stress tolerance. Through nutrient solubilization, phytohormone production, and natural immune priming, this microbial inoculant supports resilient plants in stress-prone environments or regenerative systems.

Supports natural plant defense responses (SAR/ISR)
Promotes root growth and early crop establishment
Enhances phosphorus and micronutrient availability
Contributes to soil microbial balance and biodiversity
Improves plant tolerance to drought, salinity, and heat stress

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Natural Plant Immunity Activation (SAR & ISR Pathways)

Our Bacillus subtilis soil probiotic helps plants activate their own internal defense systems against biotic and abiotic stress.

Induces Systemic Acquired Resistance (SAR) via salicylic acid signaling pathways
Triggers Induced Systemic Resistance (ISR) via jasmonic acid and ethylene pathways
Stimulates production of natural defense enzymes (e.g., peroxidase, chitinase, β-glucanase)

Root Growth, Hormonal Stimulation & Early Vigor

B. subtilis is a versatile soil inoculant that actively supports strong root systems, helping crops establish faster and grow more vigorously.

Produces indole-3-acetic acid (IAA) and other phytohormones that promote root elongation and branching
Improves root hair development for nutrient and water access
Enhances seed germination speed and early seedling vigor
Increases chlorophyll synthesis, boosting photosynthetic efficiency and biomass accumulation

Phosphorus Solubilization & Micronutrient Mobilization

This strain plays an essential role as one of the leading soil fertility microbes, especially in low-input or nutrient-deficient soils. It unlocks nutrients from the soil, improving plant availability of both macronutrients and micronutrients.

Solubilizes phosphate from insoluble forms like tricalcium phosphate, iron phosphate, and aluminum phosphate
Produces siderophores that chelate and mobilize iron (Fe³⁺) in calcareous and alkaline soils
Improves uptake of key micronutrients:
- Zinc (Zn): enzyme activation and hormone balance
- Manganese (Mn): photosynthesis and disease resistance
- Copper (Cu): lignin synthesis and stress signaling
- Magnesium (Mg): chlorophyll and energy metabolism
- Sulfur (S): amino acid and protein synthesis

Abiotic Stress Tolerance in Crops

By improving root function and physiological balance, this microbial inoculant enhances plant resilience to a wide range of environmental challenges.

Drought stress - Promotes deeper rooting and increases water-use efficiency
Salinity stress - Maintains ion balance (Na⁺/K⁺ ratio) and osmotic adjustment
Heat stress - Reduces oxidative damage by boosting antioxidant enzyme systems

Improved Soil Microbiome & Rhizosphere Stability

Recognized as one of the best microbes for soil health, B. subtilis contributes to a healthier soil microbiome by establishing early, occupying space, and producing beneficial metabolites.

Produces lipopeptides (surfactin, fengycin, iturin) that influence soil microbial dynamics
Competes for space and resources in the root zone (competitive exclusion)
Supports soil aggregation, moisture retention, and decomposition of crop residues

Application Flexibility & Cropping Compatibility

Widely used as a microbial biofertilizer in organic farming systems, Bacillus subtilis is easy to integrate into existing fertility and protection protocols.

Can be applied via seed treatment, in-furrow application, drip irrigation, fertigation, or foliar spray
Compatible with most liquid fertilizers, humic acids, and other microbial inoculants
Safe for use in conventional, no-till, and hydroponic systems
Effective across crops: row crops, vegetables, orchards, turf, and greenhouse production

Why Choose Our Bacillus subtilis?

High CFU Count for Consistent Field Performance - Formulated at 200 billion CFU/g, our Bacillus subtilis delivers robust microbial activity, rapid root colonization, and long-lasting shelf stability, ensuring reliable performance across diverse crops, soils, and growing conditions.
Supports Plant Defense Through SAR and ISR Activation - Our strain primes natural plant immunity via both Systemic Acquired Resistance (SAR) and Induced Systemic Resistance (ISR), helping crops withstand abiotic and biotic stress without chemical intervention.
Enhances Nutrient Availability and Uptake - B. subtilis solubilizes mineral-bound phosphorus and mobilizes key micronutrients such as iron, zinc, manganese, and copper, boosting overall nutrient efficiency, plant metabolism, and crop quality.
Promotes Root Development and Early Vigor - Produces phytohormones like indole-3-acetic acid (IAA) that stimulate root elongation, branching, and seedling vigor. Improves chlorophyll content and biomass accumulation for stronger, more resilient plants.
Broad Compatibility with Agricultural Inputs - Compatible with most fertilizers, humic substances, compost teas, and biological blends. Easily integrates into organic, regenerative, and conventional farming systems, including seed, soil, and foliar applications.
Trusted U.S. Producer of Beneficial Soil Bacteria - Applied Biotech Industries is a fermentation-based microbial fertilizers manufacturer supplying high-performance Bacillus subtilis in bulk, wholesale, and private-label formats. We serve distributors, input companies, and growers seeking dependable microbial inoculants for soil and plant health.

Looking to buy Bacillus subtilis for agriculture from a reliable U.S. manufacturer? Our premium B. subtilis biofertilizer is ideal for boosting crop resilience, nutrient efficiency, and rhizosphere health. Whether you're sourcing bulk soil probiotics, ordering beneficial microbes for plants, or seeking fermentation-based custom blends, our Bacillus subtilis powder for agriculture delivers proven performance in every field.

Concentration (CFU/g):

200 billion (2.0 × 10¹¹) CFU/g dry powder
Custom concentrations available upon request

Particle Size (Mesh):

Passes through 100 mesh sieve

Packaging Options:

22 lb (10 kg) pails
44 lb (20 kg) pails
340 lb (155 kg) drums
Smaller custom packaging available on request

Shelf-life:

1.5 years at room temperature
2+ years refrigerated

Storage Recommendations:

Store in a cool, dry location away from direct sunlight.
Reseal container tightly after each use.

Soil Application (Drip, Drench, or Fertigation)

Dosage:

50–100 grams per acre (125–250 grams per hectare)

Frequency:

Apply during early root establishment or transplanting
Reapply every 2–4 weeks during active growth stages as needed

Application Method:

Dissolve thoroughly in water (use enough water to reach the root zone)
Use an agitation tank or manual stirring to maintain microbial suspension
Apply through drip irrigation lines, micro-sprayers, or fertigation systems, positioning flow as close to the root zone as possible
Flush the system after application to prevent clogging or residue buildup

Do not tank mix with herbicides, fungicides, bactericides, or chemical pesticides.

In-Furrow

Dosage:

10–20 grams per acre (25–50 grams per hectare)

Frequency:

Apply once at planting to target root initiation and early growth stages.

Application Method:

Dissolve microbial powder thoroughly in water.
Apply directly into the seed furrow or planting trench at seeding time.
If using irrigation systems for delivery, ensure solution contacts the root zone.
Maintain agitation to prevent settling.

When tank-mixing with fertilizers:

Dilute fertilizer fully in water first before adding Bacillus subtilis
Do not mix with herbicides, fungicides, bactericides, or pesticides

Foliar Spray

Dosage:

25–50 grams per acre (60–120 grams per hectare)

Frequency:

Begin at vegetative or pre-flowering stages
Reapply every 7–14 days, especially during periods of high stress or disease pressure

Application Method:

Apply in the early morning or evening to reduce UV exposure
Use a non-ionic surfactant or wetting agent for improved adhesion
Ensure thorough coverage of foliage, including undersides of leaves
Agitate spray solution continuously during application

Seed Treatment

Dosage:

3–5 grams per kg of seed

Frequency:

Single application before planting

Application Method:

Mix thoroughly with a sticking agent (e.g., sugar solution, gum arabic)
Coat seeds evenly, ensuring full surface coverage
Allow seeds to dry gently in the shade before sowing

Q: What are the benefits of using Bacillus subtilis powder in agriculture?

A: Bacillus subtilis is a naturally occurring, spore-forming plant growth-promoting rhizobacterium (PGPR). It functions as a biostimulant, biofertilizer, and soil inoculant, enhancing nutrient use efficiency, stimulating root development, and priming natural plant defenses (SAR and ISR). As a trusted agricultural microbial strain, it improves soil health and crop resilience in a wide range of farming systems.

Q: What is the difference between Bacillus subtilis and other PGPR strains?

A: Bacillus subtilis stands out among plant growth-promoting rhizobacteria (PGPR) for its spore-forming ability, high stability, and broad-spectrum benefits across soil and plant systems. Unlike some PGPR that require specific environmental conditions, B. subtilis biofertilizers remain viable in diverse climates and farming systems. It enhances nutrient uptake (especially phosphorus and micronutrients), stimulates root growth, and triggers both Systemic Acquired Resistance (SAR) and Induced Systemic Resistance (ISR), making it one of the most versatile and effective microbial inoculants for agriculture.

Q: Can Bacillus subtilis be mixed with Trichoderma or mycorrhizae?

A: Yes, Bacillus subtilis is highly compatible with other beneficial microbes such as Trichoderma fungi and endomycorrhizal inoculants. These microbial species complement each other in the rhizosphere by occupying different niches. B. subtilis enhances nutrient solubilization and plant immunity, while mycorrhizae improve phosphorus and water uptake and Trichoderma supports root zone health and organic matter decomposition. Co-applying these microbial biofertilizers can amplify plant growth, resilience, and soil microbiome balance, especially in regenerative and organic farming systems.

Q: Where can I buy Bacillus subtilis in bulk?

A: Applied Biotech Industries is a direct manufacturer and fermentation producer of Bacillus subtilis powder. We supply bulk quantities from our Wisconsin-based production facility. Our wholesale packaging options are available in powder form in 22 lb pails, 44 lb pails and 340 lb drums (10 kg pails, 20 kg pails, and 155 kg drums).

Q: Does Applied Biotech Industries manufacture Bacillus subtilis?

A: Yes. We produce Bacillus subtilis in-house using controlled fermentation for high CFU counts, product stability, and consistent field performance. We’re a trusted microbial fertilizers manufacturer supplying customers across organic, regenerative, and conventional agriculture.

Q: How is Bacillus subtilis applied in agriculture?

A: Bacillus subtilis can be applied using several common methods:

Seed treatment for early root colonization
In-furrow application at planting
Soil drench or drip irrigation for root zone delivery
Foliar spray to activate natural defense responses
It is compatible with many liquid fertilizers, organic inputs, and microbial blends.

Q: What nutrients does Bacillus subtilis help plants access?

A: Bacillus subtilis improves plant access to:

Phosphorus (P) – by solubilizing mineral-bound phosphates
Iron (Fe) – through siderophore chelation
Micronutrients such as zinc (Zn), manganese (Mn), copper (Cu), magnesium (Mg), sulfur (S), and calcium (Ca)

This makes it an effective component in microbial biofertilizer programs focused on nutrient efficiency.

Q: Can Bacillus subtilis be used in certified organic farming?

A: Yes. Bacillus subtilis is a non-GMO, naturally occurring strain used widely in organic agriculture. It improves soil biodiversity, supports nutrient cycling, and enhances plant health without synthetic inputs. However, always confirm compliance with your organic certification body.

Q: Which crops benefit from Bacillus subtilis applications?

A: B. subtilis biofertilizer supports a wide range of crops, including:

Row crops – corn, wheat, soybeans, sorghum
Vegetables & fruits – tomatoes, lettuce, strawberries, citrus
Perennials – grapes, apples, berries
Specialty & high-value crops – herbs, ornamentals, greenhouse vegetables.

Q: Is Bacillus subtilis compatible with fertilizers and agricultural inputs?

A: Yes. Bacillus subtilis works well with most liquid fertilizers, humic substances, and organic inputs. When tank-mixing, dilute fertilizers in water before adding microbes. Avoid direct mixing with chemical pesticides unless compatibility has been confirmed.

Q: Is Bacillus subtilis safe for the environment?

A: Absolutely. Bacillus subtilis is a beneficial soil microbe that promotes sustainable agriculture. It improves soil fertility, reduces chemical input needs, and supports long-term productivity without environmental harm.

Q: Does Applied Biotech offer wholesale, private-label, or custom supply options?

A: Yes. We provide wholesale agricultural microbes, private-label biofertilizers, and custom formulations for agribusiness clients. Whether you need microbial inoculants for large-scale production, bulk soil probiotics, or fermentation-based formulations, we offer full technical support and flexible logistics.

Safety Data Sheet (SDS)

1. Harnessing Plant Growth-Promoting Rhizobacteria, Bacillus subtilis, to Mitigate Salt-Induced Damage in Rice (Oryza sativa L.)

🔗 Read the study

Key Findings:

Application of B. subtilis mitigated salt-induced damage in rice.
Enhanced growth parameters and stress tolerance observed in treated plants.
Improved antioxidant activity and ionic balance under saline conditions.

2. Bacillus subtilis-Mediated Abiotic Stress Tolerance in Plants

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Key Findings:

B. subtilis enhances plant tolerance to various abiotic stresses, including drought and salinity.
Mechanisms include modulation of stress-responsive genes and antioxidant enzyme activities.
Helps maintain physiological homeostasis under environmental pressure.

3. Mechanisms of Bacillus: Improving the Efficiency of Green Agriculture

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Key Findings:

Detailed mechanisms by which Bacillus species, including B. subtilis, enhance plant health.
Emphasized the role of antimicrobial compound production and induced systemic resistance.
Supports integration into sustainable and low-input farming systems.

4. Bacillus subtilis: A Plant-Growth Promoting Rhizobacterium That Also Impacts Biotic Stress

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Key Findings:

B. subtilis promotes plant growth and enhances resistance to biotic stresses.
Functions through phytohormone production, nutrient solubilization, and pathogen suppression.
Produces VOCs and bioactive peptides that influence the root microbiome.

5. Bacillus subtilis Impact on Plant Growth, Soil Health, and Environment

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Key Findings:

B. subtilis contributes to improved plant growth and soil health.
Plays a role in conferring biotic and abiotic stress tolerance to plants.
Enhances rhizosphere microbial diversity and organic matter turnover.

6. Bacillus subtilis ER-08: A Multifunctional Plant Growth-Promoting Rhizobacterium

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Key Findings:

B. subtilis ER-08 enhances plant growth and stress tolerance.
Demonstrated potential as a bio-inoculant for sustainable agriculture.
Solubilizes phosphate and produces siderophores for micronutrient mobilization.

7. Bacillus subtilis Ameliorates Water Stress Tolerance in Maize and Common Bean

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Key Findings:

B. subtilis application improved drought tolerance in maize and common bean.
Enhanced physiological and biochemical responses under water-deficit conditions.
Increased water use efficiency and maintained plant biomass under stress.

8. Bacillus subtilis Inoculation Improves Nutrient Uptake and Physiological Activity in Sugarcane Under Drought Stress

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Key Findings:

B. subtilis increased chlorophyll a and total chlorophyll by 25–30% in sugarcane, both under normal and drought conditions.
Net photosynthetic rate rose by 40% in well-watered and 67% under drought stress.
Water-use efficiency improved by 32–40%, enhancing growth and carbohydrate accumulation.

9. Yield, Nutrition, and Leaf Gas Exchange of Lettuce Plants Inoculated with Bacillus subtilis in Hydroponic Systems

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Key Findings:

A nutrient solution containing B. subtilis at 15.6–31.2 × 10³ CFU/mL increased lettuce leaf count by ~20% and fresh mass by >22%.
Stomatal conductance and photosynthetic rates improved significantly, confirming growth promotion in soilless systems.

Highlights B. subtilis’s potential as a microbial inoculant in hydroponic production.

Bacillus subtilis